Molecular Cloning and Characterization of a Locus Responsible for O Acetylation of the O Polysaccharide of Legionella pneumophila Serogroup 1 Lipopolysaccharide |
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Authors: | Chang Hua Zou Yuriy A Knirel Jürgen H Helbig Ulrich Zhringer and Clifford S Mintz |
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Institution: | Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, 33101, USA. |
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Abstract: | Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide. |
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