Conservation of cholera toxin gene in a strain of cholera toxin non-producing Vibrio cholerae O1 |
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Authors: | Yasuko Honma Masaaki Iwanaga |
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Institution: | Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan;Research Institute of Comprehensive Medicine, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-01, Japan |
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Abstract: | BT23, a Vibrio cholerae O1 El Tor isolate, possesses the cholera toxin (CT) gene as determined by PCR. However, CT was not detected in the culture medium by the reversed passive latex agglutination test, nor in the whole cell lysate as examined by Western blotting. The toxin-coregulated pilus (TCP) was not detected by Western blotting. This suggests the presence of defects in the regulatory cascade. toxR, toxS and toxT, members of the regulatory cascade, were examined by PCR. toxR and toxS were conserved but toxT was not. CT and TCP production was complemented by transformation of toxT. The lack of toxT was suspected to be the cause of the undetectable production of CT in strain BT23. |
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Keywords: | Vibrio cholerae O1 Cholera toxin productivity Polymerase chain reaction |
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