Serotonin-induced chloride secretion in hen colon. Possible second messengers |
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Authors: | M B Hansen N Bindslev |
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Institution: | Department of General Physiology and Biophysics, Panum Institute, University of Copenhagen, Denmark. |
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Abstract: | 1. Serotonin, 100 microM, induces a peak increase in short circuit current of about 150 microA/cm2 and in cord conductance of about 7 mS/cm2 and a more prolonged increase of 30 microA/cm2 and 1.4 mS/cm2 which lasts more than 30 min in hen colon. 2. The peak increase in short circuit current and cord conductance is due to a concomitant Cl- secretion. 3. The second messenger, which mediates Cl- secretion, increases in short circuit current and cord conductance, is cyclic AMP as theophylline, 0.5 mM, increases the response in short circuit current to 1 microM serotonin from 38 +/- 5 to 78 +/- 8 microA/cm2 and in g from 1.1 +/- 0.4 to 2.0 +/- 0.3 mS/cm2. 4. Theophylline, 0.5 mM, also sensitizes the hen colon to cyclic AMP yielding an EC50 of 0.24 +/- 0.03 mM in the presence of theophylline compared with an EC50 of 2.3 +/- 0.2 mM in the absence of theophylline. 5. Manipulations of other putative second messenger systems, such as the prostaglandins/leucotrienes, the phosphoinositides and external Ca2+ or calmodulin-sensitive enzymes, did not influence the serotonin response in short circuit current and cord conductance, thus ruling out their importance as intracellular mediators. |
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