A major histocompatibility locus in fish: serological identification and segregation of transplantation antigens in the common carp (Cyprinus carpio L.) |
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Authors: | Peter Kaastrup René J M Stet Albert J Tigchelaar Egbert Egberts Willem B van Muiswinkel |
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Institution: | 1. Department of Experimental Animal Morphology and Cell Biology, Agricultural University, P.O. Box 338, 6700 AH, Wageningen, The Netherlands
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Abstract: | In this study, experiments are described that were designed to investigate whether fish have an immune regulatory systems
similar to the major histocompatibility complex (MHC) in higher vertebrate species. From combinations of gynogenetic carp
showing either slow of fast rejection of skin transplants, the latter were chosen for alloantiserum production by hyperimmunization
with peripheral blood leucocytes. The resulting alloantisera were analyzed for hemagglutinating reactivity with gynogenetic
siblings and proved to be operationally monoscpecific in absorption experiments. The serologically determined carp erythrocyte
specificites were shown to correspond to two codominantly expressed allelic products of a single locus and were designated
K1 and K2, respectively. Flow cytometer analysis revealed that the same products are also present on leucocytes from peripheral
blood, thymus, spleen, and pronephros.K1-andK2-homozygous second-generation gynogenetic siblings were used to study the histocompatibiligy nature of the K locus products.
Skin transplants between K-allogeneic gynogenetic siblings were rejected significantly faster than within K-syngeneic combinations.
Taken together, these data suggest that theK locus incorporates MHC class I-like characteristics. |
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