Real-time quantitative assay of telomerase activity using the duplex scorpion primer |
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| Authors: | Yanping Huang Deming Kong Yi Yang Ruifang Niu Hanxi Shen Huaifeng Mi |
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| Affiliation: | State Key Lab of Functional Polymer Materials for Adsorption and Separation, Chemical School, Nankai University, Tianjin 300071, PR China. |
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| Abstract: | A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R2 = 0.994). The requirement of post-PCR steps is thus obviated. |
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| Keywords: | duplex scorpion primer real-time quantitative PCR telomerase activity TRAP assay |
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