Podocalyxin as a Prognostic Marker in Gastric Cancer |
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Authors: | Alli Laitinen Camilla B?ckelman Jaana Hagstr?m Arto Kokkola Christian Fermér Olle Nilsson Caj Haglund |
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Institution: | 1. Department of Surgery, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.; 2. Research Programs Unit, Translational Cancer Biology, University of Helsinki, Helsinki, Finland.; 3. Department of Pathology and Oral Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.; 4. Fujirebio Diagnostics AB, Gothenburg, Sweden.; 5. Onson Consulting, Gothenburg, Sweden.; INRS, CANADA, |
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Abstract: | BackgroundPodocalyxin-like 1 (PODXL) is a cell-adhesion glycoprotein associated with aggressive tumor phenotype and poor prognosis in several forms of cancer. The aim of this study was to investigate PODXL expression in gastric cancer by use of two different antibodies.MethodsBy tumor-tissue microarrays and immunohistochemistry we evaluated PODXL expression in tumor specimens from 337 patients who underwent surgery for gastric adenocarcinoma at Helsinki University Hospital. We used two different antibodies: HPA2110, which is a polyclonal antibody and an in-house monoclonal antibody called HES9, to investigate the association of PODXL expression with clinicopathologic variables and patient survival.ResultsPODXL staining was positive by the polyclonal antibody in 153 (57.5%) cases and by the monoclonal antibody in 212 (76%). Polyclonal antibody expression was associated with intestinal cancer type (p<0.001). Monoclonal antibody staining was associated with age over 66 (p = 0.001), with intestinal cancer (p<0.001), and with small tumor size (≤ 5 cm; p = 0.024). Both antibodies were associated with high S-phase fraction (p = 0.022; p = 0.010), and high tumor proliferation index (Ki-67; p = 0.003; p = 0.001). PODXL positivity by the polyclonal antibody indicated reduced gastric-cancer-specific 5-year survival of 24.0% (95% CI 16.9–31.1), compared to 43.3% (95% CI 33.7–52.9) for patients with PODXL negativity (p = 0.001). The result remained significant in multivariable analysis (HR = 3.17; 95% CI 1.37–7.34, p = 0.007).ConclusionIn gastric cancer, PODXL expression by the polyclonal antibody HPA2110 is an independent marker of poor prognosis. |
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