Molecular cloning and nucleotide sequencing of oxyR, the positive regulatory gene of a regulon for an adaptive response to oxidative stress in Escherichia coli: homologies between OxyR protein and a family of bacterial activator proteins |
| |
Authors: | Kazuyuki Tao Kozu Makino Shuji Yonei Atsuo Nakata and Hideo Shinagawa |
| |
Institution: | (1) Laboratory of Radiation Biology, Faculty of Science, Kyoto University, Sakyo-ku, 606 Kyoto, Japan;(2) Department of Experimental Chemotherapy, Research Institute for Microbial Disease, Osaka University, 565 Suita, Osaka, Japan |
| |
Abstract: | Summary Treatment of Escherichia coli and Salmonella typhimurium cells with a low dose of hydrogen peroxide induces expression of a large number of genes, and confers resistance to oxidative stresses. The oxyR gene encodes a positive regulatory protein for a subset of these genes involved in the defense against oxidative damage. We cloned a DNA fragment that contains the E. coli oxyR region on a plasmid vector, and analyzed the nucleotide sequence of the gene. The amino acid sequence of OxyR protein, deduced from the nucleotide sequence, shows a high degree of homology to the sequences of a number of bacterial activator proteins including LysR, cysB, IlvY, MetR and NodD. The product of the oxyR gene identified by the maxicell procedure was a 34 kDa protein, which agrees with the size predicted from the nucleotide sequence of the gene. |
| |
Keywords: | Oxidative stress Activator protein oxyR LysR family |
本文献已被 SpringerLink 等数据库收录! |
|