| 人胎盘中胶原蛋白的提取和表征 |
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| 引用本文： | 李德荣,于相梅,朱斌.人胎盘中胶原蛋白的提取和表征[J].生物加工过程,2010,8(3):58-61. |
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| 作者姓名： | 李德荣 于相梅 朱斌 |
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| 作者单位： | 1. 临沂市兰山区人民医院,临沂,276000
2. 四川职业技术学院,遂宁,629000 |
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| 基金项目： | 四川省教育厅自然科学科研基金资助项目 |
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| 摘 要： | 采用碱-酶结合法以人胎盘为原料提取胶原蛋白,并使用原子力显微镜(AFM)、差示扫描量热仪(DSC)、圆二色光谱(CD)和傅立叶变换红外光谱(FTIR)对所得胶原蛋白产品进行表征。结果表明:此工艺路线的胶原蛋白得率高,达到0.102%;提取时间由48 h以上减少到24 h以内,而且所得胶原蛋白分子完整地保留了其螺旋纤维结构。此法是从人胎盘中提取胶原蛋白的一种行之有效的方法。
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| 关 键 词： | 表征 人胶原蛋白 提取 胎盘 |
Extraction and characterization of human-placenta collagen |
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| LI De-rong,YU Xiang-mei,ZHU Bin.Extraction and characterization of human-placenta collagen[J].Chinese Journal of Bioprocess Engineering,2010,8(3):58-61. |
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| Authors: | LI De-rong YU Xiang-mei ZHU Bin |
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| Institution: | 1.People′s Hospital of Lanshan District,Linyi 276000,China;2.Sichuan Vocational and Technical College,Suining 629000,China) |
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| Abstract: | The collagen was extracted from the human-placenta by a alkali-enzyme method.The properties of the collagen were investigated by atomic force microscope（AFM） and differential scanning calorimeter（DSC）,circular dichroism（CD）,and Fourier transform infrared spectroscopy（FTIR）.The results indicated that the collagen yield was 0.102% by the new method.The extraction time was reduced from 48 h to 24 h.In addition,the collagen molecules held the fibrillar structure.The new method was effective for extracting the collagen from human-placenta. |
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| Keywords: | characterization collagen extraction human-placenta |
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