Nitrate reductase and nitrite reductase activity in free-living cells and bacteroids of Rhizobium loti |
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| Authors: | J. Monza M. J. Delgado E. J. Bedmar |
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| Affiliation: | (1) Cátedra de Bioquímica, Facultad de Agronomía, 780 Avenida Garzón, Montevideo, Uruguay;(2) Departmento de Microbiología, Estación Experimental del Zaidín, C.S.I.C., P.O. Box 419, E-18080 Granada, Spain |
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| Abstract: | Cells of Rhizobium loti strains T1 and U226 cultured in defined medium with glutamate as the only nitrogen source and bacteroids isolated from root nodules of Lotus corniculatus, L. pedunculatus and L. tenuis did not show constitutive (non-nitrate induced) nitrate reductase activity (NRA). In contrast, nitrite reductase activity (NiRA) was present in both free-living cells and bacteroids of either strain T1 or U226. Constitutive NRA and NiRA were detected in the cytosol fraction from nodules of all three symbioses examined. An induced NRA was expressed in bacteroids after a 10 h incubation in the presence of nitrate. |
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| Keywords: | bacteroids cytosol free-living cells Lotus sp. nitrate reductase nitrite reductase Rhizobium loti |
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