Soluble mediators produced by a human continuous B-lymphoblastoid cell line |
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| Authors: | R.J. Warrington W.J. Rutherford P.J. Sauder |
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| Affiliation: | Departments of Medicine & Immunology and the Rheumatic Diseases Research Laboratory, University of Manitoba, Winnipeg, Manitoba, Canada |
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| Abstract: | ![]()
The Ebstein-Barr virus-transformed human lymphoblastoid cell line PGLC-33H releases a migration inhibitory factor (MIF) of MW ~ 20,000 daltons. This MIF may appear free in serum-free culture supernatants or may be associated with a carrier material as a complex of MW ~ 60,000 daltons, from which the MIF can be dissociated. The free form of MIF possesses, or is associated with a suppressor activity for pokeweed mitogen-induced immunoglobulin synthesis. This suppressor activity is heat (56 °C) and acid pH stable but 2-mercaptoethanol sensitive and cannot be attributed to α-interferon or lymphotoxin. |
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| Keywords: | To whom all correspondence should be addressed: C319 Health Sciences General Centre 700 William Avenue Winnipeg Manitoba R3E 0Z3 Canada. |
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