| 用改进的Mg^＋＋沉淀多聚核糖体法分离胞质mRNA |
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| 引用本文： | 张西平,夏伏建.用改进的Mg^＋＋沉淀多聚核糖体法分离胞质mRNA[J].细胞生物学杂志,1998,20(1):45-47. |
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| 作者姓名： | 张西平 夏伏建 |
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| 作者单位： | 武汉大学生命科学学院 430072
(张西平，黄磊，张楚瑜，王鄂生)，中国农科院油料作物研究所 武汉(夏伏建) |
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| 摘 要： | 本文报道一种用改进的Palmiter的方法分离纯化胞质mRNA。即利用镁离子沉淀核糖体,提取细胞质总RNA,进而纯化胞质mRNA的方法。该方法得到的mRNA是正在翻译的成熟的胞质mRNA。所得mRNA得率和质量都优于其他方法,便于常规检测,并可用于cDNA的合成和PCR扩增。
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| 关 键 词： | 镁离子沉淀法 多聚核糖体 mRNA |
AN AMELIORATED METHOD FOR ISOLATION OF CYTOPLASMIC mRNA BY MEANS OF Mg PRECIPITATION OF POLYSOMES |
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| Zhang Xi Ping Huang Lei Zhang Chu Yu Wang E sheng.AN AMELIORATED METHOD FOR ISOLATION OF CYTOPLASMIC mRNA BY MEANS OF Mg PRECIPITATION OF POLYSOMES[J].Chinese Journal of Cell Biology,1998,20(1):45-47. |
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| Authors: | Zhang Xi Ping Huang Lei Zhang Chu Yu Wang E sheng |
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| Abstract: | This paper describes an ameliorated method of Palmiter for isoaltion of mRNA,who used magnesium ions to pre-cipitate polysomes, Only mature and translating mRNAs are purified by this method. The production and quality of isolated mRNAs were high and suitable for conventional analysis. The mRNA could be used for cDNA synthesis and PCR amplification. |
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| Keywords: | Magnesium pricipitation Polysomes mRNAs |
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