Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm |
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| Authors: | Kaoru Ohta Chihiro Sato Tsukasa Matsuda Masaru Toriyama Victor D Vacquier William J Lennarz Ken Kitajima |
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| Institution: | (1) Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464–8601, Japan;(2) Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka, 422–8529, Japan;(3) Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California, 92093–0202, U.S.A;(4) Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, New York, 11794–5215, U.S.A |
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| Abstract: | The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for egg ligands, a glycosylphosphatidylinositol (GPI)-anchored protein, and four proteins which may be involved in signal transduction. Co-immunoprecipitation revealed that at least three proteins, the speract receptor, the 63emsp4 ]kDa GPI-anchored protein and the  subunit of a heterotrimeric Gs protein, are localized in the LD-DIM. This suggests that the LD-DIM fraction may be a membrane microdomain for speract–speract receptor interaction, as well as the subsequent signal transduction pathway involved in induction of sperm respiration, motility and possibly the acrosome reaction. |
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| Keywords: | sea urchin sperm ganglioside detergent-insoluble membrane signal transduction fertilization acrosome reaction |
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