Chitosan enhanced gene delivery of cationic liposome via non-covalent conjugation |
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| Authors: | Bing Wang Shubiao Zhang Shaohui Cui Baoling Yang Yinan Zhao Huiying Chen Xiaomin Hao Qiong Shen Jiti Zhou |
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| Affiliation: | (1) College of Environmental and Biological Science, Dalian University of Technology, Dalian, 116024, Liaoning, People’s Republic of China;(2) SEAC-ME Key Laboratory of Biochemistry Engineering, Dalian Nationalities University, Dalian, 116600, Liaoning, People’s Republic of China; |
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| Abstract: | Two new types of stable ternary complexes were formed by mixing chitosan with DOTAP/pDNA lipoplex and DOTAP with chitosan/pDNA polyplex via non-covalent conjugation for the efficient delivery of plasmid DNA. They were characterized by atomic force microscopy, gel retarding, and dynamic light scattering. The DOTAP/CTS/pDNA complexes were in compacted spheroids and irregular lump of larger aggregates in structure, while the short rod- and toroid-like and donut shapes were found in CTS/DOTAP/pDNA complexes. The transfection efficiency of the lipopolyplexes showed higher GFP gene expression than DOTAP/pDNA and CTS/pDNA controls in Hep-2 and Hela cells, and luciferase gene expression 2–3-fold than DOTAP/pDNA control and 70–120-fold than CTS/pDNA control in Hep-2 cells. The intracellular trafficking was examined by confocal laser scanning microscopy. Rapid pDNA delivery to the nucleus enchanced by chitosan was achieved after 4 h transfection. |
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