Glycosylation of various flavonoids by recombinant oleandomycin glycosyltransferase from <Emphasis Type="Italic">Streptomyces antibioticus</Emphasis> in batch and repeated batch modes |
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Authors: | Sung Hee Choi Mili Ryu Yeo Joon Yoon Dong-Myung Kim Eun Yeol Lee |
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Institution: | (1) Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do, 446-701, Republic of Korea;(2) Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Republic of Korea;(3) Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon, 305-764, Republic of Korea; |
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Abstract: | An oleandomycin glycosyltransferase (OleD GT) gene from Streptomyces antibioticus was functionally expressed in Escherichia coli BL21 (DE3) with various molecular chaperones. The purified recombinant OleD GT catalyzed glycosylation of various flavonoids:
apigenin, chrysin, daidzein, genistein, kaempferol, luteolin, 4-methylumbelliferone, naringenin, quercetin and resveratrol
with UDP–glucose. 4.6 μg OleD GT was readily immobilized onto 1 mg hybrid nanoparticles of Fe3O4/silica/NiO on the basis of the affinity between His-tag and NiO nanoparticles with retention of 90% activity. In batch reaction,
more than 90% naringenin (20 μM) was converted to its glycoside in 5 h. The immobilized OleD GT was efficiently reused for
seven times whilst maintaining >60% of the residual activity in repeated glycosylation of naringenin. |
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