Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana?×?C. citriodora |
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Authors: | Cao Dinh Hung Stephen J Trueman |
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Institution: | (1) School of Science and Education, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia;(2) Agri-Science Queensland, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia |
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Abstract: | A protocol was developed for short-term preservation and distribution of the plantation eucalypt, Corymbia torelliana × C. citriodora, using alginate-encapsulated shoot tips and nodes as synthetic seeds. Effects of sowing medium, auxin concentration, storage
temperature and planting substrate on shoot regrowth or conversion into plantlets were assessed for four different clones.
High frequencies of shoot regrowth (76–100%) from encapsulated explants were consistently obtained in hormone-free half- and
full-strength Murashige and Skoog (MS) sowing media. Conversion into plantlets from synthetic seeds was achieved on half-strength
MS medium by treating shoot tips or nodes with 4.9–78.4 μM IBA prior to encapsulation. Pre-treatment with 19.6 μM IBA provided
62–100% conversion, and 95–100% of plantlets survived after acclimatisation under nursery conditions. Synthetic seeds containing
explants pre-treated with IBA were stored for 8 weeks much more effectively at 25°C than at 4°C, with regrowth frequencies
of 50–84% at 25°C compared with 0–4% at 4°C. To eliminate the in vitro culture step after encapsulation, synthetic seeds were
allowed to pre-convert before sowing directly onto a range of ex vitro non-sterile planting substrates. Highest frequencies
(46–90%) of plantlet formation from pre-converted synthetic seeds were obtained by transferring shoot tip-derived synthetic
seeds onto an organic compost substrate. These plantlets exhibited almost 100% survival in the nursery without mist irrigation.
Pre-conversion of non-embryonic synthetic seeds is a novel technique that provides a convenient alternative to somatic embryo-derived
artificial seeds. |
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