Nitric oxide alleviates manganese toxicity by preventing oxidative stress in excised rice leaves |
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Authors: | Sarita Srivastava R S Dubey |
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Institution: | (1) Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India; |
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Abstract: | In the present study, we have investigated the effects of nitric oxide (NO) on alleviating manganese (Mn)-induced oxidative
stress in rice leaves. Exogenous MnCl2 treatment to excised rice leaves for 24 and 48 h resulted in increased production of H2O2 and lipid peroxides, decline in the levels of antioxidants, glutathione and ascorbic acid, and increased activities of antioxidative
enzymes, superoxide dismutase, guaiacol peroxidase, catalase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione
reductase. Treatment of rice leaves with 100 μM sodium nitroprusside (SNP), a NO donor, was effective in reducing Mn-induced
increased levels of H2O2, lipid peroxides and increased activities of antioxidative enzymes. The levels of reduced ascorbate and glutathione were
considerably recovered due to SNP treatment. The effect of SNP was reversed by the addition of NO scavenger, 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide
(c-PTIO) suggesting that ameliorating effect of SNP is due to release of NO. The results indicate that MnCl2 induces oxidative stress in excised rice leaves, lowers the levels of reduced ascorbate and glutathione, and elevates activities
of the key antioxidative enzymes. NO appears to provide a protection to the rice leaves against Mn-induced oxidative stress
and that exogenous NO application could be advantageous in combating the deleterious effects of Mn-toxicity in rice plants. |
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