GABAergic disinhibition facilitates polysynaptic excitatory transmission in rat anterior cingulate cortex |
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Authors: | Wang Hua Ren Wen-Hua Zhang Yu-Qiu Zhao Zhi-Qi |
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Affiliation: | Institute of Neurobiology, Fudan University, Shanghai 200433, China. |
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Abstract: | Various studies implicate the anterior cingulate cortex (ACC) in processing pain. Combining whole-cell patch clamp recordings in rat ACC slices and a formalin-induced conditioned place avoidance (F-CPA) behavioral model, the present study was to address the effect of GABA(A) receptors on excitatory transmission to ACC layer V neurons and its possible functional significance related to pain. Removal of GABA(A) inhibition by bicuculline (10 microM) induced a novel long-lasting response in layer V neurons, which could be blocked by high divalent extracellular solution and was sensitive to relatively higher rate stimuli. Co-application of NMDA receptor antagonist APV (50 microM) and non-NMDA receptor antagonist DNQX (10 microM) completely blocked the responses. Enhancement of inhibition by intra-ACC microinjection of muscimol abolished the acquisition of F-CPA without affecting formalin-induced acute nociceptive responses. These results suggest that GABA(A) inhibition may be involved in pain-related aversion by modulating glutamate-mediated excitatory transmission in the ACC. |
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Keywords: | GABAA receptor Polysynaptic response Conditioned place avoidance Anterior cingulate cortex |
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