Assay of proteolytic enzyme activity using a 14C-labeled hemoglobin |
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Authors: | J S Roth T Losty E Wierbicki |
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Affiliation: | Section of Biochemistry and Biophysics, Biological Sciences Group, University of Connecticut, Storrs, Connecticut 06268 USA; Food Laboratory, U. S. Army Natick Laboratories, Natick, Massachusetts 01760 USA |
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Abstract: | The measurement of total tissue protcolytic activity with a hemoglobin substrate labeled with K14CNO is described. The labeled hemoglobin is stable and its use offers several advantages, particularly in specificity and sensitivity over methods commonly in use. |
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