Determination of pyrimidine dimer unwinding angle by measurement of DNA electrophoretic mobility |
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| Authors: | G Ciarrocchi A M Pedrini |
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| Affiliation: | Istituto di Genetica Biochimica ed Evoluzionistica, C.N.R. Pavia, Italy |
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| Abstract: | The unwinding angle produced by the formation of one pyrimidine dimer has been estimated to be ?14.3 ° ±0.2 °. This value has been obtained by titrating the number of pyrimidine dimers necessary to reduce the number of superhelical turns by one in each topoisomer obtained by treatment of a supercoiled DNA with DNA topoisomerase I. |
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