Differential expression of fibrillar collagen genes during callus formation |
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| Authors: | P Multim?ki H Aro E Vuorio |
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| Affiliation: | 2. Macquarie University, Sydney, NSW, Australia;1. Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya, Barcelona-Tech (UPC), Campus Diagonal Besòs-EEBE, Av. Eduard Maristany 10, 08019 Barcelona, Spain;2. CIEFMA-Department of Materials Science, Universitat Politècnica de Catalunya, Barcelona-Tech (UPC), Campus Diagonal Besòs-EEBE, Barcelona 08019, Spain;3. Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, Barcelona-Tech, Campus Diagonal Besòs-EEBE, Barcelona 08019, Spain |
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| Abstract: | An experimental fracture healing model in the rat tibio-fibular bone was employed to study the appearance of messenger RNAs for types I, II and III collagens during endochondral fracture repair. Total RNA was extracted from normal bone and from callus tissue at various time points. The total RNAs were analyzed in Northern hybridization for their contents of procollagen mRNAs using specific cDNA clones. The results show that during the first week of fracture repair type III collagen mRNA is increased to the greatest extent, followed by type II collagen mRNA during the second week. The 28-day callus resembles bone by containing mainly type I collagen mRNAs and very little type II or III collagen mRNA. |
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