卵巢催乳素受体mRNA在小鼠卵泡发育及排卵过程中的表达

目的探讨卵巢催乳素受体(Prolactin receptor,PRLR)mRNA在小鼠不同发育阶段及排卵过程中的表达情况。方法选择不同发育时期的昆明小鼠,以及性未成熟昆明小鼠予以PMSG-HCG(Pregnancy Mare Serum Gonadotro-phin-Humane chorionic gonadotrophin,孕马血清促性腺激素-人绒毛膜促性腺激素)序贯处理,采用实时定量聚合酶链反应(Real-Time Polymerase Chain Reaction,Real-Time PCR)检测小鼠卵巢中PRLR mRNA的表达。结果PRLR mR-NA的表达水平随小鼠的不断发育而显著性升高,PRLR在6周龄小鼠卵巢中的表达量是3周龄小鼠的3.86倍(P0.01),11周龄与33周龄小鼠的表达水平分别是3周龄小鼠的19.67倍、19.81倍(P0.01);PMSG处理后12h,PRLR表达水平是对照组的1.44倍(P0.05),24h、48h后分别达到5.48倍和7.14倍(P0.01),HCG处理后4h、8h、12h,PRLR表达水平有所下降,但仍高于对照组(P0.01),24h、48h后其表达水平再次升高,分别是对照组的5.64倍和6.04倍。结果 PRLR对小鼠卵泡的生长、发育及其黄体形成与维持发挥重要作用。

EXPRESSION OF OVARIAN PROLACTIN RECEPTOR mRNA DURING FOLLICULAR DEVELOPMENT AND OVULATION IN THE MOUSE

Objective To explore the expression of prolactin receptor（PRLR） mRNA in the different developmental stages of the mouse ovary and the procedure of ovulation.Methods Mouse ovaries of different developmental stages were collected.The immature mice were chosen to be treated with PMSG and HCG sequentially and the ovaries were collected at various time.The expression of PRLR was detected by real time polymerase chain reaction（Real-Time PCR）.Results The expression of PRLR was increased with the development of the mouse.PRLR expression of 6-week-old mice was 3.84 fold of 3-week-olds（P0.01）.The expression levels of 11 and 33-week-olds were 19.67 and 19.81 fold respectively of 3-week-olds（P0.01）.The expression of PRLR was increased after 12h of PMSG treatment（P0.05）and 5.48 and 7.14 fold respectively of the control after 24h and 48h（P0.01）.After the sequential treatment of HCG,the expression level was significantly decreased but still higher than that of the control until 24h（P0.01）.PRLR expression at 24h and 48h post-HCG treatment was 5.48 and 7.14 fold of the control respectively（P0.01）.Conclusion PRLR may be involved in folliculogenesis and play an important role in the formation and maintenance of corpus luteum in the mouse ovary.