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Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor |
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| Authors: | Sapsford Kim E Taitt Chris R Fertig Stephanie Moore Martin H Lassman Michael E Maragos Chris M Shriver-Lake Lisa C |
| Affiliation: | aGeorge Mason University, 10910 University Blvd, MS 4E3, Manassas, VA 20110, USA bCenter for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA cMycotoxin Research Unit, USDA-ARS-NCAUR, 1815 N, University Street, Peoria, IL 61604, USA |
| Abstract: | Because of the potential health risks of aflatoxin B1 (AFB1), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB1 in buffer, corn and nut products. AFB1-spiked foods were extracted with methanol and Cy5-anti-AFB1 added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned with immobilized AFB1. The resulting fluorescence signal decreased as the concentration of AFB1 in the sample increased. The limit of detection for AFB1 in buffer, 0.3 ng/ml, was found to increase to between 1.5 and 5.1 ng/g and 0.6 and 1.4 ng/g when measured in various corn and nut products, respectively. |
| Keywords: | Array biosensor Indirect competitive immunoassay Aflatoxin B1 Corn products Nut products |
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