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Molecular Characterization of Three Heritiera Species Using AFLP Markers
Authors:A.K. Mukherjee  L.K. Acharya  I. Mattagajasingh  P.C. Panda  T. Mohapatra  P. Das
Affiliation:(1) DNA Fingerprinting Laboratory, Department of Plant Biotechnology, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, 751015 Orissa, India;(2) Department of Taxonomy and Conservation, Regional Plant Resource Centre, Nayapalli, Bhubaneswar, 751015 Orissa, India;(3) DNA Fingerprinting and Rice Genome Laboratory, National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India
Abstract:In the present study three species of Heritiera Aiton (Sterculiaceae) were characterized using 9 amplified fragment length polymorphism (AFLP) primer combinations and the genetic relationship among these three species was assessed, Nine AFLP primer combinations yielded 445 bands out of which 210 were monomorphic and 235 were polymorphic. Out of the 235 polymorphic bands 79 were present only in a single species. Among the total amplified bands 255 were shared between H. fomes and H. littoralis, 225 were shared between H. fomes and H. macrophylla and 306 bands were shared between H. littoralis and H. macrophylla. The cluster analysis showed H. littoralis is closer to H. macrophylla than H. fomes. The similarity between H. fomes and H. littoralis was higher than that of H. fomes and H. macrophylla. The present study indicates that H. littoralis is better classified as mangrove associate or back mangal than a true mangrove. This revised version was published online in July 2006 with corrections to the Cover Date.
Keywords:cluster analysis  mangroves  monomorphic and polymorphic bands
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