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| 焦化废水中4株苯酚高效降解菌的分离及鉴定 | |
| 引用本文： | 周林,朱爽,陈木华,吴海珍,韦朝海.焦化废水中4株苯酚高效降解菌的分离及鉴定[J].生物技术,2010,20(2):44-46. |
| 作者姓名： | 周林 朱爽 陈木华 吴海珍 韦朝海 |
| 作者单位： | 1. 广东药学院生命科学与生物制药学院,广东广州,510006 2. 华南理工大学生物科学与工程学院,广东广州,510006 3. 华南理工大学环境科学与工程学院,广东广州,510006 |
| 基金项目： | "十一五"国家科技支撑计划重点项目，国家高技术研究发展计划(863)项目，国家自然科学基金资助项目 |
| 摘 要： | 目的:从焦化废水中筛选苯酚高效降解菌并进行鉴定.方法:在100～1000 mg/L的苯酚为惟-碳源的无机盐培养基上分离出单菌落,测定各菌株的生长曲线以及对苯酚的降解效牢;利用 16S rDNA序列分析结合菌株的形态特征确定各菌株的分类地位.结果:筛选获得4株苯酚降解菌,均能够以苯酚为惟一碳源,在30℃、pH7.0、摇床转速130 r/min、2%的接种量条件下,24h内能将1 000mg/L的苯酚降解91%以上;4株菌可初步鉴定为芽孢杆菌属(ZL1)、产碱杆菌属(ZL2、ZL4)、沙雷氏菌属(ZL3).其中,从焦化废水中分离出高效降解苯酚的沙雷氏菌未见报道.结论:从焦化废水中获得4株苯酚高效降解细菌,对高浓度含酚废水的生物降解具有潜在的应用前景. |
| 关 键 词： | 苯酚 生物降解 焦化废水 |
Isolation and Identification of Four Highly Effective Phenol-degrading Strains from Coking Wastewater |
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| ZHOU Lin,ZHU Shuang,CHEN Mu-hua,WU Hai-zhen,WEI Chao-hai.Isolation and Identification of Four Highly Effective Phenol-degrading Strains from Coking Wastewater[J].Biotechnology,2010,20(2):44-46. | |
| Authors: | ZHOU Lin ZHU Shuang CHEN Mu-hua WU Hai-zhen WEI Chao-hai |
| Institution: | ZHOU Lin1,ZHU Shuang1,CHEN Mu-hua1,WU Hai-zhen2,WEI Chao-hai3(1.School of Life Science , Biopharmacology,Guangdong Pharmaceutical University,Guangzhou 510006,2.School of Biological Science , Engineering,South China University of Technology,Guangzhou,Guangdong 510006,3.College of Environmental Science , Engineering,Guangdong,China) |
| Abstract: | Objective:To isolate and identify highly efficient phenol-degrading strain from coking wastewater.Method:The microbes were isolated by the inorganic salt medium containing 100-1 000mg/L phenol as a sole carbon source in order to obtain efficient phenol-degrading strain.The growth curve and phenol-degrading efficiency was determined,also the taxonomic statuses of these strains were determined based on morphological features and 16S rDNA sequences analysis.Result:Four highly effective phenol-degrading bacteri... |
| Keywords: | 16S rDNA phenol biodegradation 16S rDNA coking wastewater |
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