急性运动所致线粒体某些功能的改变及胆红素的保护作用

目的：探讨急性运动所致疲劳的机理以及胆红素的保护作用。方法：将Wistar大鼠随机分为对照组、运动组、运动恢复组、胆红素处理运动组、胆红素处理运动恢复组,共5组,分别灌胃1μmol/Kg体重的胆红素或生理盐水4周,负重（体重的5％）游泳2h后处死,测定有关指标。结果：急性运动后即刻大鼠排肠肌胞、线粒体Ca^2 含量明显升高,恢复12h后线粒体Ca^2 含量呈继续升高的趋势;线粒体C^a2 -Mg^2 -ATP酶、Ca^2 -ATP酶活性明显下降,12h后有所回升,胆红素处理后可以明显抑制这些指标的改变,但线粒体Mg^2 -ATP酶活性的变化在胆红素处理组和未处理组间无明显差异,均明显低于对照组,只是胆红素组的恢复相对较快。结论：生理浓度的胆红素可能通过抑制线粒体某些功能的改变而保护细胞名受急性运动所致的损伤,从而延缓疲劳的发生,加速恢复。

Changes of the certain functions of mitochondria induced by acute exercise and the protective effect of bilirubin

To study the mechanism of fatigue induced by acute exercise and the protective effect of bilirubin. Methods: 41 Wistar male rats we re divided into five groups: control group,exercise group, exercise recovery gro up,bilirubin-treateol exercise group anol bilirubin-treateol exercise recover y group.The rats were administrated with 1 μmol/kg body weight of bilirubin or sa line once every day for 4 weeks, after swimming with load for 2 h, all of the ra ts were killed and several index were determined. Results: Acute exe rcise could induce the increase of Ca2+ content in cytoplasm and mitochond ria of gastrocnemius, Ca2+ content increased continuously after 12 hours i n mitochondria. The ca2+-Mg2+-ATPase activity decreased obviously after acute exercise, and the activity increased again after 12 hours. Bilirubin could inhibit these changes. The change of Mg2+- ATPase activity in mito chondria had little difference between bilirubin-treated and untreated groups, both of which were lower than that of control group, but the recovery was faster in the bilirubin-treated group than that in untreated group. Conclusio n: Bilirubin could protect muscle fibers from injury induced by acute ex ercise and delay the development of fatigue and promote the recovery through inh ibiting the disturbance of the certain function of mitochondria.