Verteilung und Aufteilung der DNS bei einigen Cyanophyceen,festgestellt durch ihre DAPI-Fluoreszenz |
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Authors: | Univ-Prof Dr Elisabeth Tschermak-Woess Alfred Schöller |
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Institution: | 1. Institut für Botanik der Universit?t Wien, Rennweg 14, A-1030, Wien, ?sterreich
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Abstract: | The DNA of Blue-Green Algae may be specifically and most efficiently elucidated by the combined use of the fluorochrome DAPI
and DNAse treatment. InChroococcus turgidus andSynechococcus aeruginosus it forms a network which usually is positioned at the periphery of the centroplasm. At the beginning of cell division it
seems to be invaginated by the ingrowing cell wall. Later it must be distributed by other means because two equal parts are
connected by a number of fine and straight DNA threads. InOscillatoria limosa usually all parts of the centroplasm are interspersed with the DNA network. In surface view, the DNA occasionally seems to
consist of a number of independant rods, but in reality these are connected to the general network. Division apparantly occurs
by ingrowth of the crosswall and usually results in equal daughter nuclear equivalents although occasionally they are unequal.
Four strains ofMicrocoleus vaginatus were found to have nuclear equivalents of the same general appearance as inOscillatoria limosa.
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Keywords: | Cyanophyceae Chroococcus turgidus Merismopedia glauca Synechococcus aeruginosus Oscillatoria limosa Microcoleus vaginatus — Nuclear equivalent distribution and partition of the DNA-network in cell division DAPI-fluorescence |
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