Ca2+-induced fragmentation of actin filaments in pollen tubes |
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Authors: | T Kohno T Shimmen |
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Institution: | (1) Department of Botany, Faculty of Science, University of Tokyo, Hongo, 113 Tokyo, Japan |
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Abstract: | Summary To control the intracellular free Ca2+ concentration from the cell exterior, pollen tubes ofLilium longiflorum were treated with a Ca2+ ionophore, A23187. Cytoplasmic streaming was inhibited when the free Ca2+ concentration of the external medium (Ca2+]) was raised to 5×10–6 M or higher. At Ca2+] below 1×10–6 M, the rhodamine-phalloidin stained actin filaments appeared straight and thin. However, at Ca2+] which inhibited cytoplasmic streaming, the actin filaments appeared fragmented. In pollen tubes, Ca2+ regulation of cytoplasmic streaming may be linked not only to myosin (Shimmen 1987) but also to actin.Abbreviations ATP
adenosine-5-triphosphoric acid
- Ca2+]
concentration of free Ca2+
- EGTA
ethyleneglycol-bis-(-aminoethylether)N,N,N,N-tetraacetic acid
- HEPES
N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid
- PIPES
piperazine-N,N-bis(2-ethanesulfonic acid)
- Rh-ph
rhodamine-conjugated phalloidin |
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Keywords: | Actin filament Ca2+ Cytoplasmic streaming Lilium longiflorum Pollen tube |
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