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Development of antler-type fruiting bodies of Ganoderma lucidum and determination of its biochemical properties
Authors:Surya Sudheer  Zain Taha  Sivakumar Manickam  Asgar Ali  Poh Guat Cheng
Affiliation:1. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Malaysia;2. Centre of Excellence for Postharvest Biotechnology (CEPB), School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Malaysia;3. Ganofarm Sdn. Bhd, Taman Pelangi, 42800, Tanjung Sepat, Selangor, Malaysia
Abstract:
Following the importance of antler-type fruiting bodies of Ganoderma lucidum, in this study, the impact of main growth parameters such as ventilation and light on the development of antler-type fruiting bodies has been investigated together with the determination of physico-chemical properties of antler fruiting bodies. For this, the primordia bags of G. lucidum were kept under controlled ventilation to adjust the CO2 produced by the mushrooms owing to its respiration under light and dark conditions. The bioactive compounds such as phenolics, flavonoids, water-soluble polysaccharides and ganoderic acid showed a two-fold increase in the antler-type fruiting bodies as compared to normal kidney-shaped fruiting bodies. It is assumed from this study that the antler type fruiting bodies are developed due to restricted ventilation which causes an increase in the level of CO2 gas in the air as a result of respiration of mushroom. The shape and colour of antler fruiting bodies again dependent on the light provided in the growth chamber. This study also proves that with the manipulation of light and ventilation antler-type fruiting bodies of G. lucidum could be developed with higher quantity of bioactive compounds and with higher antioxidant potential.
Keywords:Antioxidant  Bioactive compounds  Growth conditions  Lingzhi  Mushroom  PDA  potato dextrose agar  BE  biological efficiency  TPC  total phenolic content  DPPH  2,2-diphenylpicrylhydrazyl  FRAP  Ferric reducing ability of plasma  ABTS  2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)  TBARS  thiobarbituric acid reactive substances  MDA  malondialdehyde  RPM  revolutions per minute  GAE  gallic acid equivalent  ROS  reactive oxygen species
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