Hydrogen-rich water mediates redox regulation of the antioxidant system,mycelial regeneration and fruiting body development in Hypsizygus marmoreus |
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| Authors: | Hui Chen Haibo Hai Hong Wang Qian Wang Mingjie Chen Zhiyong Feng Ming Ye Jinjing Zhang |
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| Institution: | 1. Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China;2. National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, China;3. Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, China;4. College of Life Science, Nanjing Agricultural University, No.1, Weigang Road, XuanWu District, Nanjing, 210095, China |
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| Abstract: | Hypsizygus marmoreus is an important industrialized mushroom, yet the lack of basic research on this fungus has hindered further development of its economic value. In this study, mycelia injured by scratching were treated with hydrogen-rich water (HRW) to investigate the involvement of the redox system in fruiting body development. Compared to the control group, damaged mycelia treated with HRW regenerated earlier and showed significantly enhanced fruiting body production. Antioxidant capacity increased significantly in damaged mycelia after HRW treatment, as indicated by higher antioxidant enzyme activities and antioxidant contents; the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) were also reduced at the mycelial regeneration stage after treatment with HRW. Furthermore, genes involved in ROS, Ca2+, MAPK and oxylipin signaling pathways were up-regulated by HRW treatment. In addition, laccase and manganese peroxidase activities and mycelial biomass were higher after HRW treatment, suggesting that HRW might enhance the substrate-degradation rate to provide more carbon sources for fruiting body production. |
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| Keywords: | Fruiting body Hydrogen-rich water Mycelial regeneration ROS balance HRW hydrogen-rich water ROS reactive oxygen species MAPK mitogen-activated protein kinase SOD superoxide dismutase GPX glutathione peroxidase GR glutathione reductase CAT catalase GSH glutathione AsA ascorbic acid MDA malonaldehyde superoxide radical hydrogen peroxide |
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