|
|||||
|
|
Frequency of angiotensin II type 1 receptor gene polymorphism in Turkish acute stroke patients |
|
| Authors: | Kurt Hulyam Bayramoglu Aysegul Gunes Hasan Veysi Ozbabalik Demet Degirmenci Irfan Colak Ertugrul Cosan Turgut Didem Bayram Banu Dikmen Miris |
| Institution: | 1. Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University, , Eskisehir, Turkey;2. Department of Biology, Faculty of Science and Art, Artvin Coruh University, , Artvin, Turkey;3. Department of Neurology, Faculty of Medicine, Eskisehir Osmangazi University, , Eskisehir, Turkey;4. Department of Biostatistics, Faculty of Medicine, Eskisehir Osmangazi University, , Eskisehir, Turkey;5. Medical Laboratory Skills, Vocational School of Health Services, Mugla S?tk? Kocman University, , Marmaris, Mugla, Turkey;6. Department of Pharmacology, Faculty of Pharmacy, Anadolu University, , Eskisehir, Turkey |
| Abstract: | This study was performed in acute stroke patients in the Turkish population to determine the frequency of the A1166C polymorphism in the AT1 gene and to examine the role of this polymorphism in acute stroke development. In this study, 257 genomic DNA samples were analysed (from 206 acute stroke patients and 51 healthy individuals). Genomic DNA was prepared from peripheral blood using the salt‐extraction method. The presence of the A1166C polymorphism in the AT1 gene was determined using the polymerase chain reaction (PCR)‐restriction fragment length polymorphism (RFLP) method. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge‐coupled device (CCD) camera. In this study, the allele frequency at the A1166C position was 92% A and 8% C for control and 97% A and 3% C for patients. This difference in allele frequency between the control group and the patient group was not statistically significant. However, genotype and allele frequencies showed a significant difference (P < 0.001) in the control and the patient groups. The results of this study show no relationship between the A1166C polymorphism in the AT1 gene and acute stroke in the Turkish population. |
| Keywords: | acute stroke AT1 gene A1166C polymorphism |