Identification and characterization of potential NBS-encoding resistance genes and induction kinetics of a putative candidate gene associated with downy mildew resistance in <Emphasis Type="Italic">Cucumis</Emphasis> |
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Authors: | Hongjian Wan Zhenguo Zhao Ahmed Abbas Malik Chuntao Qian Jinfeng Chen |
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Institution: | (1) State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China |
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Abstract: | Background Due to the variation and mutation of the races of Pseudoperonospora cubensis, downy mildew has in recent years become the most devastating leaf disease of cucumber worldwide. Novel resistance to downy
mildew has been identified in the wild Cucumis species, C. hystrix Chakr. After the successful hybridization between C. hystrix and cultivated cucumber (C. sativus L.), an introgression line (IL5211S) was identified as highly resistant to downy mildew. Nucleotide-binding site and leucine-rich
repeat (NBS-LRR) genes are the largest class of disease resistance genes cloned from plant with highly conserved domains,
which can be used to facilitate the isolation of candidate genes associated with downy mildew resistance in IL5211S. |
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