Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila |
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| Authors: | B Petridou M F Guerin F Hayes |
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| Institution: | Laboratoire de Chimie Cellulaire, Institut de Biologie Physico-Chimique, Paris, France. |
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| Abstract: | Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks. |
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