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Culture of asparagus protoplasts on porous polypropylene membrane
Authors:Yan Kong  Chee-Kok Chin
Affiliation:(1) China Rice Institute, Hanzou, People's Republic of China;(2) Department of Horticulture and Forestry, Cook College, Rutgers University, 08903 New Brunswick, NJ, USA
Abstract:A method of using a buoyant porous polypropylene membrane floated on liquid medium to culture protoplasts of Asparagus officinalis L. is described. This method supports very good growth and eliminates the need to periodically replenish culture medium lost to evaporation.Abbreviations BAP 6-Benzyl aminopurine - 2, 4-D, 2, 4 Dichlorophenoxyacetic acid - 2iP N-(3-Methyl-2-butenyl)-1H-purin-6-amine - NAA Naphthaleneacetic acid
Keywords:
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