Culture of asparagus protoplasts on porous polypropylene membrane |
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Authors: | Yan Kong Chee-Kok Chin |
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Affiliation: | (1) China Rice Institute, Hanzou, People's Republic of China;(2) Department of Horticulture and Forestry, Cook College, Rutgers University, 08903 New Brunswick, NJ, USA |
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Abstract: | A method of using a buoyant porous polypropylene membrane floated on liquid medium to culture protoplasts of Asparagus officinalis L. is described. This method supports very good growth and eliminates the need to periodically replenish culture medium lost to evaporation.Abbreviations BAP 6-Benzyl aminopurine - 2, 4-D, 2, 4 Dichlorophenoxyacetic acid - 2iP N-(3-Methyl-2-butenyl)-1H-purin-6-amine - NAA Naphthaleneacetic acid |
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