传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长的抑制作用

目的：通过动物实验探讨传输靶向COX-2siRNA联合化疗药物对大鼠胃癌细胞生长的抑制作用。方法：24只健康SD大鼠平分为三组，治疗组用COX-2-siRNA转染的胃癌SGC7901细胞接种，同时进行环磷酰胺、丝裂霉素C化疗治疗；阴性对照组，用阴性对照siRNA转染的胃癌SGC7901细胞接种，同时进行环磷酰胺、丝裂霉素C化疗治疗；对照组（n=8），用未经转染的胃癌SGC7901细胞接种，不进行化疗治疗；三组转染后都接种了裸鼠。结果：治疗组、阴性对照组及对照组胃癌细胞凋亡率分别为（22．28±0．12）％、（1．23±0．17）％和（1．03±0．14）％，治疗组与阴性对照组和对照组比较差异都有统计学意义（t=18．152，17．555，P〈0．05）。治疗组的抑瘤率为76．7％，阴性对照组和对照组分别为12．8％和6．89％，治疗组的抑瘤率明显高于其他两组（x^2=15．211，13．899，P〈0．05）。Western blotting检测结果显示治疗组的COX-2蛋白表达含量得到了明显抑制。结论：传输靶向COX-2 siRNA和化疗药物的配合应用可有效抑制COX-2蛋白的表达，从而抑制胃癌细胞的生长，从而起到更好的治疗效果。

Effects of COX2 siRNA Combined with Chemotherapeutics on the Inhibition of Gastric Cancer Cells in Rats

Objective： To investigate the effects of COX2 siRNA combined with chemotherapeutics on the inhibition of gastric carcinoma cells of rats. Methods： 24 healthy SD rats were selected equally divided into three groups, which including the treatment group （n=8） that were given the SGC7901 cells with COX-2-siRNA transfection and treated by the cyclophosphamide and mitomycin C in chemotherapy; the negative control group （n=8） that were given the negative control siRNA transfected cells with gastric cancer SGC7901, and treated by the cyclophosphamide and the mitomycin C in chemotherapy; the control group （n=8） that were given the untransfected SGC7901 without the chemotherapy; Then the mice in the three groups were vaccinated. Results： The rate of the cells＇ apoptosis in the three groups was （22.28± 0,12）%, （1.23± 0.17）% and （1.03± 0.14）% , respectively with statistically significant differences （t=18.152, 17.555, P〈0.05）. The rate of antitumor in the three groups was 76.7 %, 12.8% and 6.89%, respectively. The inhibition rate in the treatment group was significantly higher than those of the other two groups （X^2=15.211,13.899, P〈0.05）. Western blotting analysis showed that COX-2 protein expression levels in the treatment group was significantly inhibited. Conclusion： Transmission targeting COX-2 siRNA combined with the chemotherapy drugs can effectively inhibit the expression of COX-2 protein, thereby inhibite the growth of gastric cancer cells, which played better therapeutic effect.