Studies on formulation of a combination heat killed immunogen from diarrheagenic Escherichia coli and Vibrio cholerae in RITARD model |
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| Institution: | 1. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, 24 Chocimska, 00-791 Warsaw, Poland;2. Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159 C, 02-787 Warsaw, Poland;3. Department of Bacteriology, National Institute of Public Health—National Institute of Hygiene, 24 Chocimska, 00-791 Warsaw, Poland;4. Department of Medical Biology, The Cardinal Stefan Wyszyński Institute of Cardiology, Alpejska 42, 04-628 Warsaw, Poland;1. Illawarra Health and Medical Research Institute and School of Chemistry and Molecular Bioscience, Molecular Horizons, University of Wollongong, Wollongong, New South Wales, Australia;2. W. M. Keck Center for Transgene Research, Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, United States;3. School of Chemistry and Molecular Bioscience, Australian Infectious Diseases Research Centre, University of Queensland, St. Lucia, Queensland, Australia;1. Centre for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Centre, University of Lausanne, Lausanne, Switzerland;2. School of Public Health, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheeva, Israel;3. Members of the Board of the European Study Group for Genomic and Molecular Diagnostics (ESGMD) |
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| Abstract: | Multiple diarrheagenic enteric bacterial infections cause global morbidity and mortality. A combination vaccine is needed to combat different diarrhea-causing organisms. In our present work, we formulated a combination of antigens from three different diarrheagenic Escherichia coli strains and three different Vibrio cholerae strains. We demonstrated that our newly formulated combination immunogen was able to raise species-specific immunogenicity. This formulation also gave protection against different diarrheagenic E. coli strains in the removable intestinal tie-adult rabbit diarrhea model. However, protective efficacy was not found against the V. cholerae El Tor Ogawa Haitian variant, but challenged with V. cholerae El Tor Inaba or O139 showed protection in rabbits.This is the first report of a single formulated nonliving heat-killed combination immunogen from different diarrheagenic E. coli and V. cholerae that could bestow protection against different bacteria in an animal model. |
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| Keywords: | Heat-killed RITARD |
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