Influence of green light illumination on several enzymes involved in energy metabolism in the oriental armyworm,Mythimna separata (Lepidoptera: Noctuidae) |
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| Institution: | 1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China;2. State Academy of Sciences, Institute for Biodiversity, Pyongyang 00850, Democratic People''s Republic of Korea;3. Pyong Yang Medical College, Kim Il Sung University, Pyongyang 00850, Democratic People''s Republic of Korea;1. Key Laboratory of Functional Foods, Ministry of Agriculture/Guangdong Key Laboratory of Agricultural Products Processing/Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China;2. School of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China;3. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China;1. Insect Biosystematics Laboratory, Seoul National University, Seoul 151-921, Republic of Korea;2. Animal Phylogeny and Systematics Lab., Department of Biology, Kunsan National University, Gunsan 573-701, Republic of Korea;3. Department of Plant Medicine and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Republic of Korea;4. Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea;5. Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea;1. Department of Zoology, Government Arts College, Melur, 625106 Madurai, Tamil Nadu, India;2. Centre for Research in Aquatic Entomology, The Madura College, Madurai 625011, Tamil Nadu, India;3. Department of Biochemistry, School of Life Sciences, Central University of Rajasthan, Rajasthan 305801, India;1. Laboratory of Insect Biosystematics, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea;2. Department of Plant Medicine and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea;1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan 410128, China;2. College of Plant Protection, Hunan Agricultural University, Changsha, Hunan 410128, China.;3. Deparment of Forensic Science, College of Medical Medicine, Central South University, Chansgha, Hunan 410013, China |
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| Abstract: | The oriental armyworm, Mythimna separata Walker, is an important insect pest in eastern Asia. Green (520 nm) light with an exposure time of >30 min can result in a stronger phototactic response than the wavelengths of other lights in M. separata moths. The present study was mainly focused to estimate the activities of several enzymes (such as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycerol-3-phosphate dehydrogenase (GPDH), lactate dehydrogenase (LDH) and 3-hydroxyacyl-CoA dehydrogenase (HOAD)) involved in the energy metabolism and a ratio of HOAD to GAPDH activity in M. separata moths exposed to the green light for different exposure times. Our results showed that when M. separata moths were exposed to the green light for 30 min, the activities of the two enzymes (GPDH and HOAD) and as well as the ratio of HOAD to GAPDH activity were significantly elevated. The activity of LDH was significantly increased in the moths exposed for 60 min. Furthermore, significant differences in enzyme activities between the male and female moths were recorded in 45 min exposure time group of the GAPDH, all light treatment groups of the LDH, and 45 min group of the ratio of HOAD to GAPDH activity, respectively. We suggest that when M. separata adult moths are exposed to the green light, these enzymes can be activated to produce energy for starting the phototactic behavior to the green light. Our findings may provide a theoretical basis for elucidating a reason of the phototactic behavior of nocturnal moths. |
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