Fermentation and isolation of herbicolin A,a peptide antibiotic produced byErwinia herbicola strain A 111 |
| |
Authors: | M. Greiner G. Winkelmann |
| |
Affiliation: | (1) Institut für Biologie I, Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 1, W-7400 Tübingen, Federal Republic of Germany |
| |
Abstract: | Summary
Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A.
Offprint requests to: G. Winkelmann |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|