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Fermentation and isolation of herbicolin A,a peptide antibiotic produced byErwinia herbicola strain A 111
Authors:M. Greiner  G. Winkelmann
Affiliation:(1) Institut für Biologie I, Mikrobiologie I, Universität Tübingen, Auf der Morgenstelle 1, W-7400 Tübingen, Federal Republic of Germany
Abstract:Summary Erwinia herbicola (Enterobacter agglomerans), belonging to the Enterobacteriaceae, produces the lipopeptide antibiotics herbicolin A and B, which are active against sterol-containing fungi. Fermentation of these antibiotics was performed in 20-1 stirred glass fermentors in a batch process. Best yields of antibiotic production were found at low cultivation temperatures in a TRIS-buffered chemically defined medium. Under these conditions the amount of impurities aggravating the purification was minimized. Isolation was performed by adsorption, and gel and ion exchange chromatographic techniques. In a final purification step preparative high performance liquid chromatography (PHPLC) yielded pure herbicolin A. Offprint requests to: G. Winkelmann
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