Factors Affecting Germination of Oospores of Phytophthora infestans |
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| Authors: | T T Chang W H Ko |
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| Institution: | Taiwan Forest Research Institute, 53 Nan Hai Road, Taipei, Taiwan (R.O.C.);Department of Plant Pathology, Beaumont Agricultural Research Center, University of Hawaii, Hilo, HI 96720 (U.S.A.). |
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| Abstract: | When oospores from the pairing between A1 and A2 mating types of Phytophthora infestans were treated with 0.25 % KMnO4 solution for 15 min and incubated at 19 °C under light on a modified S+L medium, germination commenced within 4 days and reached about 70 % after 20 days. Under these conditions, more than 25 % of oospores obtained from a 4-day-old culture germinated. To obtain a high germination rate of P. infestans oospores, light was essential during germination but not during growth and oospore formation. The optimum time for activation of oospores with 0.25 % KMnO4 was 15 to 30 min and a suitable concentration of KMnO4 for 15 min activation was 0.25 to 0.45 %. |
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