NAG11和NAG12基因转染对鼻咽癌细胞生长的影响

为了考察鼻咽癌表达下调／缺失基因NAG11和NAG12对鼻咽癌细胞系HNE1生长的影响，构建了NAG11和NAG12基因真核表达载体pcDNA3.1（＋）／NAG11和pcDNA3.1（＋）／NAG12，采用脂质体转染技术将真核重组质粒和空载体质粒分别导入HNE1细胞，观察转染后HNE1细胞生物学特性的变化，结果显示，NAG11重表达对HNE1细胞生长和细胞周期没有明显的影响，而NAG12重表达对HNE1细胞有生长抑制作用，与空载体转染组相比，倍增时间由24.1h延长至31.1h，停滞于G0－G1期细胞数由51.42%增加至68.14%。以上实验进一步说明鼻咽癌是多基因改变的疾病，NAG12的重表达有助于处国咽癌恶性表型的逆转。

Effect of NAG11 and NAG12 Gene Transfection on the Growth of Nasopharyngeal Carcinoma Cells

To study the effect of NAG11 and NAG12 gene on NAG11- and NAG12-negative nasopharyngeal carcinoma (NPC) cell line HNE1, the pcDNA3.1( )/NAG11 and pcDNA3.1( )/NAG12 mammalial expressing recombination were constructed and were tranfected into HNE1 cells, then cytobiologic characterization of the transfected HNE1 cells was detected by population double time (PDT) and flow cytometric analysis. The results showed that NAG11 reexpression did not play obvious role on the cell growth of HNE1, while NAG12 reexpression had definite growth suppression on HNE1, such as a longer PDT and an accumulation of cells G1 phase. These data further demonstrated that NPC is a disease involving polygenic alteration and NAG12 gene could favor malignant phenotype reversion of NPC cells.