Structural basis for restriction-site polymorphism at the albumin locus in inbred strains of rats |
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Authors: | Andras Gal Jean-Louis Nahon Gerard Lucotte Tamas Erdos José M Sala-Trepat |
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Institution: | (1) Laboratoire d'Enzymologie du Centre National de la Recherche Scientifique, 91190 Gifsur-Yvette, France;(2) Present address: Laboratoire de Génétique Moléculaire du CNTS, 75015 Paris, France |
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Abstract: | Two types of variant EcoRI restriction enzyme patterns of albumin-gene DNA fragments are found in different inbred strains of rats and reflect allelic polymorphism. The structural basis of the two allelic forms has been analyzed by mapping the EcoRI fragments using cloned albumin cDNA probes corresponding to the 5 or 3 end of the rat albumin mRNA and different genomic subclones. Additional restriction fragment length polymorphism has been detected using the restriction endonucleases HindIII and MspI. The results suggest that the two allelic variants differ from each other by multiple cleavage-site variations (base-pair substitutions) and by an insertion or deletion of DNA sequences. An extensive DNA sequence variation appears to exist between the two forms of the albumin gene; we have estimated that as much as 4% of the nucleotides in this region varied between the two alleles. All of this genetic variation is found in the intervening sequences of the gene and has no phenotypic manifestation.This work was supported by grants from the Institut National de la Santé et de la Recherche Médicale (Contract 7940 222) and the Association pour le développement de la Recherche sur le Cancer (Contract 6109). A. Gal was supported by a fellowship from the Institut National de la Santé et de la Recherche Médicale.On leave of absence from Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary. |
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Keywords: | albumin gene DNA evolution DNA sequence variation inbred rat strains restriction-site polymorphism |
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