DNA Profiling and Assessment of Genetic Relationships Among Important Seedless Grape (Vitis vinifera) Varieties in India Using ISSR Markers

In the present work, 43 seedless grape (Vitis vinifera) varieties were evaluated using 14 informative ISSR primers. A total of 119 bands were scored, out of which 79% were polymorphic. The level of polymorphism varied according to the primers which was evident also from different Resolving Power values of the individual primers. Based on the DNA fingerprint data of only three primers (UBC 857, 888 and 890), all the varieties except one could be identified. UPGMA cluster analysis revealed high degree of similarity among the varieties. Distinct subgroups based on the berry colour were observed. No intravarietal differences were detected. Overall grouping was in accordance with morphological characters and pedigree, proving usefulness of ISSR markers to assess genetic relationships among grape varieties.