Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus |
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| Authors: | Hatsumi Taniguchi Shigeo Kubomura Hideaki Hirano Kumiko Mizue Midori Ogawa Yasuo Mizuguchi |
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| Affiliation: | Department of Microbiology, School of Medicine, University of Environmental and Occupational Health, Fukuoka, Japan. |
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| Abstract: | A new thermostable hemolysin (delta-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had no homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela. |
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| Keywords: | DNA-cloning Sequencing Hemolysin, a new Vibrio parahaemolyticus |
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