Structure of polysaccharide-peptidoglycan complex from the cell wall of Lactobacillus casei YIT9018 |
| |
| Authors: | M Nagaoka M Muto K Nomoto T Matuzaki T Watanabe T Yokokura |
| |
| Affiliation: | Yakult Central Institute for Microbiological Research, Tokyo. |
| |
| Abstract: | The isolation and analysis of the polysaccharide-peptidoglycan complexes of Lactobacillus casei YIT9018 are presented. Two polysaccharide-peptidoglycan complexes, PS-PG1 and PS-PG2, were solubilized from the heat-killed cell by treatment with N-acetylmuramidase. PS-PG1 was composed of glucose, rhamnose, and small amount of galactose and glucosamine. PS-PG2 was composed of glucose, rhamnose, galactosamine, and glucosamine. The ratio by weight of these fractions was about 1:8. PS-PG2 was analyzed in detail. Smith degradation and deamination of this complex yielded oligosaccharide units. The results of methylation analysis of these units and intact PS-PG2 led to the most probable structure of PS-PG2: (formula; see text) |
| |
| Keywords: | |
|
|
