Ethylene formation in Pisum sativum and Vicia faba protoplasts |
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Authors: | Guy Micha Kende Hans |
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Institution: | (1) MSU-DOE Plant Research Laboratory, Michigan State University, 48824 East Lansing, MI, USA;(2) Present address: Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer, Israel |
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Abstract: | Protoplasts isolated from leaves of peas (Pisum sativum L.) and of Vicia faba L. produced 1-aminocyclopropane-1-carboxylic acid (ACC) from endogenous substrate. Synthesis of ACC and conversion of ACC to ethylene was promoted by light and inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and carbonyl cyanide m-chlorophenylhydrazone. Aminoethoxyvinylglycine inhibited ethylene synthesis to a minor extent when given during incubation of the protoplasts but was very effective when added both to the medium in which the protoplasts were isolated and to the incubation medium as well. Radioactivity from U-14C]methionine was incorporated into ACC and ethylene. However, the specific radioactivity of the C-2 and C-3 atoms of ACC, from which ethylene is formed, increased much faster than the specific radioactivity of ethylene. It appears that ACC and ethylene are synthesized in different compartments of the cell and that protoplasts constitute a suitable system to study this compartmentation.Abbreviations ACC
1-Aminocyclopropane-1-carboxylic acid
- AVG
aminoethoxyvinylglycine
- CCCP
carbonyl cyanide m-chlorophenylhydrazone
- DCMU
3-(3,4-dichlorophenyl)-1,1-dimethylurea |
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Keywords: | 1-Aminocyclopropane-1-carboxylic acid Aminoethoxyvinylglycine Ethylene synthesis Pisum (ethylene synthesis) Vicia |
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