Low-temperature fluorescence emission changes in thylakoids induced by acetyl glyceryl ether phosphorylcholine (AGEPC)

The electroneutral zwitterionic phosphatidylcholine derivative 1-0-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AGEPC), when added to low-salt chloroplasts (Tricine-washed), induces an enhancement of the F685/F730 ratio in the 77 degrees K fluorescence emission spectrum. The effect depends highly on AGEPC concentration and AGEPC/Chl ratio. Phosphatidylcholine with similar electrical properties is ineffective. AGEPC, when added to low-salt plastids of intermittent light plants, also induces changes in the 77 degrees K emission spectrum (decrease in the long wave length 720-nm band and blue-shift of the 681-nm band). The effect is attributed to modulation of the organization of the photosynthetic units via the AGEPC incorporation in the thylakoid, rather than to charge effects.