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Callus culture ofPterocladia capillacea (Rhodophyta) and analysis of cell wall polysaccharides |
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| Authors: | Xue-Wu Liu Cyrille Rochas Bernard Kloareg |
| Institution: | 1. School of Biological Sciences, Victoria University of Wellington, Private Bag, Wellington, New Zealand 2. Centre d'Etudes Océanologiques et de Biologie Marine, CNRS UPR 4601 et Université Paris VI, BP 74, F-29680, Roscoff, France 3. Centre d'Etudes et de Recherches sur les Macromolécules Végétales, CNRS, UPR 5301, P.B. 53, F-38042, Grenoble cédex, France |
| Abstract: | Calluses induced fromPterocladia capillacea have been kept in culture for more than three years. They exhibit a fast growth rate, owing to the release of single cells, which in turn develop into new callus. The effect of various media and culture conditions upon growth was investigated. In order to confirm the identity of the callus cells, a 0,45 mg incoculum was grown that yielded 15 g dried callus within six weeks. Polysaccharides from this material (5.5 g) were analysed by13C NMR spectroscopy. This produced a spectrum typical of agar and very similar to the one obtained for agar extracted fromP. capillacea plants. However, the callus agar displayed no gel-forming properties, even after alkali modification.author for correspondence |
| Keywords: | callus culture agar 13C NMR spectroscopy Pterocladia capillacea |
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