Study of thrombopoietin for gene therapy of thrombocytopenia |
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Authors: | CHONG Song LU Daru LI Changben QIU Xinfang XUE Jinglun |
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Institution: | (1) Institute of Genetics, Fudan University, 200433 Shanghai, China |
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Abstract: | Thrombopoietin (TPO) is likely to be a potent, specific and reliable medication in the treatment of throm-bocytopenia. A TPO-highly-expressed plasmid pcDNA3-TPO was constructed and a primary study was made on the expression of TPO cDNA in vitro and gene transfer study for thrombocytopenia in vivo. rhTPO showed complete and stable bioactivity by a series of indicators. High expression of TPO was detected in plasma from healthy mice or thrombocytopenia mice model receiving direct intramuscular injection of pcDNA3-TPO. And the platelet level of healthy mice peaked to 1.9-fold of baseline. Mice with CTX- induced thrombocytopenia achieved profound nadirs, acceleration of recovery, even 1.8-2.0-fold supranormal levels of peripheral platelet counts. The results offered experimental support for clinical application of gene therapy for thrombocytopenia via direct intramuscular injection of TPO cDNA. |
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Keywords: | thrombopoietin (TPO) gene transfer thrombocytopenia intramuscular injection |
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