| 杆状病毒表达系统及其应用进展 |
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| 引用本文： | 韦永龙,李轶女,张志芳,沈桂芳.杆状病毒表达系统及其应用进展[J].生物技术通报,2010(10). |
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| 作者姓名： | 韦永龙 李轶女 张志芳 沈桂芳 |
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| 作者单位： | 中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | 国家自然科学基金项目(30770279).国家"863"计划项目 |
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| 摘 要： | 杆状病毒是节肢动物门的专性寄生病毒,20世纪80年代被开发为表达载体以来,由于其真核表达环境等优点,受到了广泛的重视和研究,短短不到30年的时间里,杆状病毒表达体系的重组载体构建技术,筛选技术得到了很大程度的改进和简化,成为与大肠杆菌、酵母、哺乳动物相并列的四大表达体系之一。在表面展示,类病毒颗粒表达,哺乳动物基因转移,RNA干扰等方面取得了重要的成果。就杆状病毒表达系统的发展及其应用作一个综述。
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| 关 键 词： | 杆状病毒 表达 |
Advances in Research and Application of Baculovirus Expression System |
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| Wei Yonglong,Li Yinv,Zhang Zhifang,Shen Guifang.Advances in Research and Application of Baculovirus Expression System[J].Biotechnology Bulletin,2010(10). |
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| Authors: | Wei Yonglong Li Yinv Zhang Zhifang Shen Guifang |
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| Institution: | Wei Yonglong Li Yinv Zhang Zhifang Shen Guifang(Biotechnology Research Institute,CAAS,Beijing 100081) |
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| Abstract: | Baculoviruses are obligate lethal pathogens of arthropodas.They have been highly valued and widely studied since being exploited as a expression vector in 1980s,for their excellent advantage including eukaryotic expression enviroment.In less than 30 years,great advances have achieved involving recombinant baculovirus construction and screening.Thus,the baculovirus expression vector system(BEVS)has become one of the four most popular expression system comprising bacterium,yeasts,mammalian cells and baculovir... |
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| Keywords: | BEVS |
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