Regulation of the plasma membrane during exposure to low temperatures in suspension-cultured cells from a cryophyte (Chorispora bungeana) |
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Authors: | Yulan Shi Lizhe An Manxiao Zhang Chenghong Huang Hua Zhang Shijian Xu |
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Institution: | (1) Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou;(2) School of Life Sciences, Lanzhou University, Lanzhou |
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Abstract: | Summary. As the outermost boundary of the cell, the plasma membrane plays an important role in determining the stress resistance of
organisms. To test this concept in a cryophyte, we analyzed alterations of several components in plasma membranes isolated
from suspension-cultured cells of Chorispora bungeana Fisch. & C.A. Mey in response to treatment at 0 and −4 °C for 192 h. When compared with the controls growing at 25 °C, both
the membrane permeability and fluidity showed recovery after the initial impairment. Linolenic acid and membrane lipid unsaturation
increased by about 0.8-fold following cold treatments, although the kinetics of the increase varied with the temperatures
examined. During the treatments, the plasma membrane H+-ATPase (EC 3.6.1.3) activity increased by 78.06% at 0 °C and 100.47% at −4 °C. However, the plasma membrane NADH oxidase
(EC 1.6.99.3) activity only decreased when exposed to a lower temperature (−4 °C), and remained at 63.93% after being treated
for 192 h. After the treatments, the physical properties of the plasma membranes of suspension-cultured cells, especially
the −4 °C treated cells, were similar to those in the wild plants. These findings indicate that the specific mechanism of
cold resistance of C. bungeana is tightly linked with the rapid and flexible regulation of membrane lipids and membrane-associated enzymes, which ensure
the structural and functional integrity of the plasma membrane that is essential for withstanding low temperature.
Correspondence: Lizhe An, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences,
Lanzhou 730000, People’s Republic of China. |
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Keywords: | : Chorispora bungeana Low temperature Plasma membrane Plasma membrane H+-ATPase Plasma membrane NADH oxidase |
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