Curcumin prevents Cr(VI)-induced renal oxidant damage by a mitochondrial pathway |
| |
Authors: | Molina-Jijón Eduardo Tapia Edilia Zazueta Cecilia El Hafidi Mohammed Zatarain-Barrón Zyanya Lucia Hernández-Pando Rogelio Medina-Campos Omar Noel Zarco-Márquez Guillermo Torres Ismael Pedraza-Chaverri José |
| |
Institution: | a Department of Biology, Faculty of Chemistry, National Autonomous University of Mexico, 04510 University City, D.F., Mexicob Department of Nephrology, National Institute of Cardiology “Ignacio Chávez,” 14080 Mexico, D.F., Mexicoc Department of Biochemistry, National Institute of Cardiology “Ignacio Chávez,” 14080 Mexico, D.F., Mexicod Department of Cardiovascular Biomedicine, National Institute of Cardiology “Ignacio Chávez,” 14080 Mexico, D.F., Mexicoe Experimental Pathology Section, National Institute of Medical Sciences and Nutrition “Salvador Zubirán,” 14000 Mexico, D.F., Mexicof Animal Care Unit, Faculty of Medicine, National Autonomous University of Mexico, 04510 University City, D.F., Mexico |
| |
Abstract: | We report the role of mitochondria in the protective effects of curcumin, a well-known direct and indirect antioxidant, against the renal oxidant damage induced by the hexavalent chromium Cr(VI)] compound potassium dichromate (K2Cr2O7) in rats. Curcumin was given daily by gavage using three different schemes: (1) complete treatment (100, 200, and 400 mg/kg bw 10 days before and 2 days after K2Cr2O7 injection), (2) pretreatment (400 mg/kg bw for 10 days before K2Cr2O7 injection), and (3) posttreatment (400 mg/kg bw 2 days after K2Cr2O7 injection). Rats were sacrificed 48 h later after a single K2Cr2O7 injection (15 mg/kg, sc) to evaluate renal and mitochondrial function and oxidant stress. Curcumin treatment (schemes 1 and 2) attenuated K2Cr2O7-induced renal dysfunction, histological damage, oxidant stress, and the decrease in antioxidant enzyme activity both in kidney tissue and in mitochondria. Curcumin pretreatment attenuated K2Cr2O7-induced mitochondrial dysfunction (alterations in oxygen consumption, ATP content, calcium retention, and mitochondrial membrane potential and decreased activity of complexes I, II, II-III, and V) but was unable to modify renal and mitochondrial Cr(VI) content or to chelate chromium. Curcumin posttreatment was unable to prevent K2Cr2O7-induced renal dysfunction. In further experiments performed in curcumin (400 mg/kg)-pretreated rats it was found that this antioxidant accumulated in kidney and activated Nrf2 at the time when K2Cr2O7 was injected, suggesting that both direct and indirect antioxidant effects are involved in the protective effects of curcumin. These findings suggest that the preservation of mitochondrial function plays a key role in the protective effects of curcumin pretreatment against K2Cr2O7-induced renal oxidant damage. |
| |
Keywords: | BHT butylated hydroxytoluene BUN blood urea nitrogen CAT catalase CCCP m-chlorophenylhydrazone CDNB 1-chloro-2 4-dinitrobenzene CUR curcumin DNPH 2 4-dinitrophenylhydrazine DPPH 2 2-diphenyl-1-picrylhydrazyl radical EDTA ethylenediaminetetraacetic acid G6PDH glucose-6-phosphate dehydrogenase GPx glutathione peroxidase GR glutathione reductase GSH glutathione reduced form GSSG glutathione oxidized form GST glutathione S-transferase Hepes N-(2-hydroxyethyl)piperazine-N&prime -(2-ethanesulfonic acid) HPLC high-pressure liquid chromatography 4-HNE 4-hydroxy-2-nonenal HO-1 heme oxygenase 1 MDA malondialdehyde NAC N-acetylcysteine NAG d-glucosaminidase" target="_blank">N-acetyl-β-d-glucosaminidase NBT nitroblue tetrazolium NQO1 NADPH quinone oxidoreductase 1 Nrf2 nuclear factor-E2-related factor 2 Pi inorganic phosphate PMS phenazine methosulfate RC respiratory control index ROS reactive oxygen species SOD superoxide dismutase TCA trichloroacetic acid TMPD N&prime N&prime N&prime N-tetramethyl-p-phenylendiamide |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|